Coding

Part:BBa_J70569:Design

Designed by: Julie Norville   Group: Knight Lab   (2010-04-11)

Expressed N-terminal split GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Remove BsaXI site.


Source

Pcr'd from J04430 with BsaXI site removed.

J70569f:

gtttcttcgaattcgcggccgcttctagaggattcattaatgcagctggcacgac

J70569r:

tcttcctgcagcggccgctactagtattattattgtttgtctgccatgatgtatacattg

Melting temperature ~55 degrees, so PCR at 52 degrees C

References